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In vitro pull-down assay showed that GSTP-S184D displayed a stronger affinity to bind HMGB1 than GSTP (WT), but GSTP-S184A did not combine with HMGB1, indicating that Ser184 phosphorylation was necessary for the association between GSTP and HMGB1 (Figures 5H,I)

Acknowledgements This work was supported by the National Science Centre, Poland (NCN) (grant number: 2021/05/X/NZ9/00243)

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