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Catalytic activity of total, cytosolic, and crude membrane fractions isolated from MCF7-vector and MCF7-GSTP1 CDNB is a well established and commonly used GST substrate [3] that is conjugated with GSH to form 2,4-dinitrophenyl-S-glutathione (DNP-SG)

Since glutathione is something your body already produces, taking supplements typically doesn't cause serious side effects

Oncotarget 9, 2457224573

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GPx1, which contains a selenocysteine (Sec) residue at its active site, is irreversibly inactivated by its own substrate as the result of the oxidation of selenium atom followed by the conversion of oxidized Sec to dehydroalanine (DHA)