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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

Before each injection, check that the solution is clear and colourless (or slightly yellowish for some products), free from particles or cloudiness, within its expiry date and in-use period, and that the pen is undamaged