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The first step was to determine the best chromatographic conditions for the analysis, the selection of a mobile phase, duration of analysis, the amount of sample to be analyzed, determination of the smallest detectable concentration of the analyte, retention time for GSH and GSSG, limit of detection and quantification, the choice of chromatographic column, and the selection of operating parameters of HPLC instrument (1200 series HPLC system

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While the exact sequence of events mediated by Dsg-specific antibodies is not fully clear, three pathways for the induction of acantholysis have been proposed: (i) direct interference with Dsg transinteraction (steric hindrance)