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After revitalization, the inoculum was serially diluted 100 times to obtain a working culture containing 89 log CFU mL 1 as determined by plate counts following Niccolai et al

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Up-regulation of the PPP flux provides cells with R5P for nucleotide biosynthesis, as well as NADPH for biosynthesis and maintaining redox homeostasis ( 6PGL is found to be associated with shorter overall survival in breast cancer patients with bone metastases ( Elevated 6PGD and its product Ru5P inhibit AMPK activation by disrupting the active LKB1 complex, which promotes lipogenesis by abolishing AMPK-dependent acetyl-CoA carboxylase 1 (ACC1) phosphorylation and inactivation ( Elevated 6PGD expression and/or enzyme activity is also found in liver cancer, cervical cancer, thyroid cancer, breast cancer, ovarian cancer, non-small cell lung cancer (NSCLC) and leukemia ( Enzymes in the Non-oxidative PPP RPI and RPE expression is induced by the oncogenic Kras mutation, which is critical for the initiation of PDAC

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