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Alkylated proteins were incubated with either 10 g trypsin (4 h at 37C) in ABC buffer, or 10 g pepsin (1 h at 37C) with added 1% formic acid in 10% MeOH to obtain pH 2 during digestion

Similarly, bone density assessments are vital, as they can reveal indicators of osteoporosis and facilitate preventive or remedial measures

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