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Following the isolation of FACS-sorted cells, both Sielaff et al., and Alfredsson et al., carried out a wash step with PBS before lysis, which was effective at removing most unwanted contaminants, such as residual BSA (Bovine Serum Albumin) and EDTA (Ethylenediaminetetraacetic acid) present in the FACS buffer 62,63

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dynamic range of approximately 56 orders of magnitude , depending on sample complexity and instrument type

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Sharps container (optional) Remove the caps Sanitize the rubber stoppers Attach the needle Draw the bac water Pull back on the plunger to draw your desired volume of bacteriostatic water

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