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In the first case, this is due to the fact that NEM does not react with GSH under acidic conditions

Source numerical data and unprocessed blots are provided

While minor non-EV proteins may still be present, subsequent analyses focused on proteins consistently enriched in EV fractions across all samples to minimize potential confounding effects on proteomic profiling

doi: 10.1016/j.abb.2008.02.007
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