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429 Peptide-targeting subcellular organelles The structural integrity and functional stability of organelles are essential for maintaining normal cellular physiological functions

For the targeted analysis 8 fmol of Cytochrome C digest was added to the samples before injection in LC-MS/MS (Thermo Scientific)

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[DOI] [PMC free article] [PubMed] [Google Scholar] Stadtman ER

To further validate whether RanBPM physically interacts with p21 protein, we performed GST-pull down assay by using the purified recombinant His-RanBPM and GST-p21 proteins