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As a primary screening, genomic DNAs from the tail of the pups were subjected to genomic PCR to identify animals with the transgene using the three primer pairs covering different parts of the construct (pair A: pCAGGS_FW2 : 5TAACCATGTTCATGCCTTCT3 and Mpst_Rv1 : 5TTTACTGAGCCAGGGATGT3, pair B: Mpst_Fw1 : 5ATCTACGACGACAGTGACCA3and globinA_Rv1 : 5GTCGAGGGATCTCCATAAGA3, and pair C: CAG_Fw4 : 5ACCTGGGTCGACATTGATTA3 and CAG_Rv2 : 5AACATGGTTAGCAGAGGCTC3)

The treatment is recommended for those who: are experiencing a lack of energy due to poor diet or overwork

Cell 91 , 443446 (1997)

doi: 10.1038/s41467-017-01970-x, 70

While p53 modulators, including FOXO4-DRI, MDM2 inhibitors, and USP7 inhibitors have shown senolytic effects by selectively eliminating senescent cells or promoting their apoptosis in the laboratory, translating these findings to the clinic have so far been unsuccessful