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occasionally oxyhemoglobin loses one electron (23% per day) leading to the production of O 2 and oxidized Hb (metHb) (Figure 2) which is not able to bind and carry O 2

It maintained retinal integrity [11], recovered pupil function [11,12], and recovered retinal ischemia [13]

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This involved mixing 6 L of calibrator or sample with 600 L of triglyceride reagent and incubating at 37C for 3 min