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Experimental studies performed with cultured rat pulmonary microvascular ECs demonstrated that exposure to tobacco smoke condensate causes increased transcription and activity of the ROS generating enzyme xanthine dehydrogenase/oxidase [75]
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Briefly, the authors studied the GPx-like activity of ebselen under cell-free conditions using GSH-reduced nicotinamide adenine dinucleotide (NADH)-GR coupled assay