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Specific gene expression was quantified by real-time PCR performed on a LightCycler 480 Instrument (Roche Diagnostics Ltd., Rotkreuz, Switzerland)

Given that lipidation of apoE is favored when the protein is in its monomeric form [84], it is possible that the reduced hydrophobicity and/or conformational change in apoE3 caused by the V236E substitution results in its reduced oligomerization and increased lipidation capacity, contributing to its protective role in AD

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