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Sensorgram data were double referenced (subtraction of running buffer alone and flow cell 1) and fit to a 1:1 binding kinetics fitting model in Biacore evaluation software (Biacore Insight Evaluation Software Version 5.0.18.22102) to yield association and dissociation rate constants k on and k off , respectively

Behavioral tests The motor coordination of the mice was assessed by rotarod test, pole test, and gait analysis, as described previously (5, 43)

Hao L, Guo Y, Peng Q, et al

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