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The membranes were incubated with Bax (1:2000, 50599-2-Ig, Proteintech, United States), Bcl-2 (1:500, 26593-1-AP, Proteintech, United States), Caspase-3 (1:500, 19677-1-AP, Proteintech, United States), p-PI3K (1:500, Bs-5570R, Proteintech, United States), and p-AKT (1:500, 66444-1-Ig, Proteintech, United States) at 4C overnight

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