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The peripheral venous hematocrit and hemoglobin concentration did not reflect the hypovolemic state and the significant reductions in the red blood cell volume and plasma volume

To terminate the cultures, 20 L of 0.1% Triton X-100 and 30 L of lyticase (1 mg/mL in 0.1 M phosphate buffer, pH 7.5, containing 50 mM mercaptoethanol) were added, and the plates were incubated for 1 h at 35C
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