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Practical considerations for determination of the thiol/disulfide redox state of proteins Gel shift assays are frequently used to determine the thiol/disulfide redox status of a protein

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Derewenda, U., Derewenda, Z., Dodson, E.J., Dodson, G.G., Reynolds, C.D., Smith, G.D., Sparks, C., and Swenson, D
u , 3-hydroxytetradecanedioic acid

This operation can be expressed equivalently in vector form as: where w T denotes the transpose of the weighting vector: For the present sampling protocol, where the intervals are 4 h, 5 h, and 7 h, this becomes: and therefore: This vector formulation is mathematically identical to trapezoidal integration of the circadian cortisol curve and makes explicit that the ASI profile represents a weighted temporal integration of hormone concentrations determined solely by the sampling schedule
