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Filter-binding assays To measure intrinsic affinity of ZSWIM8 for RNAs, a dilution series of purified ZSWIM8-3xHA was incubated in excess with radiolabelled RNAs in 18 mM HEPES pH 7.4, 150 mM KOAc, 1 mM Mg(OAc) 2 , 5% (v/v) glycerol, 0.01% (v/v) IGEPAL CA-630, 0.25 mM BME, 0.25 mM TCEP (Supelco, 646547), 0.1 mg ml 1 recombinant albumin and 0.01 mg ml 1 yeast tRNA, at 25 C for 2 h

[DOI] [PMC free article] [PubMed] [Google Scholar] Yang H, Wang W, Zhang Y, Zhao J, Lin E, Gao J, He J

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miR-146a has been identified as a promising diagnostic biomarker due to its association with neurogenesis, abnormal brain anatomy, and memory deficits [169]

A population-based sample of 4365 adults was followed up after 11 years, including questionnaires, spirometry and DNA blood sampling
