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Briefly, 2 days post-confluent 3T3-L1 preadipocytes incubated with DMEM containing 10% FBS, 1 M dexamethasone (Sigma-Aldrich), 0.5 mM 1-methyl-3-isobutylxanthine (Sigma-Aldrich) and 5 g/mL insulin (Sigma-Aldrich)