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Doyle and Salvemini, 2021

Normalization counts of reads were done by mapping reads per kilobase of transcript per million (RPKM) to annotated Ensembl genes using the open-source software Bioconductor easyRNASeq v1.6.0 package on R version 3.0 (Lucent Technologies, Inc., Murray Hill, NJ)

Some of those parameters change abruptly upon a transition from gel to fluid phase, which would in total produce an abrupt change of the peak shift at the point of phase transition

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