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it contributes to the formation of the protective covering around nerves, known as the myelin sheath, and its deficiency can cause neurological issues like numbness and tingling in the hands and feet

We maintained these cells in culture for up to 2 months until assays were performed

This consistent schedule is key to preventing your levels from dipping again

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker
