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We were unable to test treatments containing gemcitabine in the 3D set-up, because prolonged incubation with gemcitabine (necessary for invasion studies through collagen matrices), resulted in the death of all tumour cells on the top of the 3D matrix

Zhao, Z., Pei, Z., Hu, A., Zhang, Y

That not only reduces the potential benefits but can also lead to unwanted side effects

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In some cases, both may be used together depending on your needs
