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Quadruplicates were seeded in poly-l-lysine (0.01%) pre-coated Seahorse XF96 Cell Culture Microplates and the plate was kept in a non-carbonated incubator for 30 min at 37 C

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[link] Liu X, Grogan T, Hieronymus H, Hashimoto T, Mottahedeh J, Cheng D, Zhang L, Huang K, Stoyanova T, Park JW, Shkhyan RO, Nowroozizadeh B, Rettig MB, Sawyers CL, Elashoff D, Horvath S, Huang J, Witte ON, Goldstein AS, "Low CD38 Identifies Progenitor-like Inflammation-Associated Luminal Cells that Can Initiate Human Prostate Cancer and Predict Poor Outcome", Cell Reports 17 (10): 2596-2606 (2016)