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2.3.2 Erythrocyte lysate EDTA blood mixed with cold distilled water was centrifuged (10,000 x g, 10 min, 4C, Rovall RC6+, Thermo Fisher Scientific, Dreieich, Germany) and the top layer forming the erythrocyte lysate (erylysate) was stored at -80C before analysis

Additional info on zinc

The prevalence of CHE was reported to be between 30% and 84% in patients with cirrhosis [7,12,13]

Therefore, it is recommended that natural agents should be used as adjunctive therapy for prevention and alleviating migraine attacks

(25(11)):913-916 doi:10.1016/j.jiac.2019.04.020
