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Dot blot Total RNA was isolated by Trizol Reagent (R701-01, Vazyme, China) and then mixed with a triploid RNA incubation buffer formulated with formamide, methanol, and 10 MOPS

Melanoma cells were also identified in these studies based on DsRed, which was stably expressed in all melanomas along with luciferase

Final Tips Use clean hands and alcohol wipes before every injection Never use a peptide that looks cloudy, discolored, or has visible particles Rotate injection sites to avoid irritation Affiliate Disclosure: This blog may include affiliate links

[DOI] [PMC free article] [PubMed] [Google Scholar] 28.Legouis D, Ricksten SE, Faivre A, Verissimo T, Gariani K, Verney C, et al

2022;11(1) doi: 10.1530/ETJ-21-0024