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After filtration, centrifugation, precipitation, and resuspension, FLS were cultured in complete culture media (DMEM with 10% fetal bovine serum, 1% antibiotics) and maintained at 37 C in a humidified incubator of 5% CO 2 and 95% air

These reductions in the NMR peak intensity were caused by a decrease in free amino groups on CAR due to the ionic interaction with HSC

However, our study did not elucidate how lipid droplets affect inflammation and apoptosis during liver regeneration, which will be further investigated in the future

Does diacylglycerol accumulation in fatty liver disease cause hepatic insulin resistance

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