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The steps below come from the DailyMed retatrutide label and standard subcutaneous injection technique from MSKCC

Overall safety profile: Meta-analysis found no significant increase in overall adverse events compared to placebo [6][7]

After centrifugation, the supernatant was pre-cleared with protein-G Dynabeads (ThermoFisher, Waltham, MA) before the immunoprecipitation experiment

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