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5-7 Multiple studies have linked gut dysbiosis a change or imbalance in the diversity, composition and functions of the microbiota to reduced GLP-1 levels

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Varin EM, Mulvihill EE, Baggio LL et al (2019) Distinct neural sites of GLP-1R expression mediate physiological versus pharmacological control of incretin action

The cells were exposed to a range of treatments: TGF-1 (10 ng/ml, R&D Systems Inc., Minneapolis, USA), IL-1 (10 ng/ml, R&D Systems Inc., Minneapolis, USA), MCC950 (10 M, Selleck, Houston, TX, USA), 3PO (10 or 30 M, Selleck, Houston, TX, USA), liraglutide (10, 100 or 1000 nM, Selleck, Houston, TX, USA), and sodium L-lactate (20 mM, Sigma-Aldrich, St