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This essentially means that, whereas agonist binding induces TM6 opening in a class A GPCR with simultaneous G a interaction and almost instant nucleotide release, i.e., G a subunit activation, agonist binding alone at a class B GPCR induces G a subunit interaction without inducing, necessarily, TM6 opening, which is a prerequisite for the guanine nucleotide exchange on the G a subunit, i.e., G protein activation, to occur [7]

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McCartney CR, Blank SK, Prendergast KA, Chhabra S, Eagleson CA, Helm KD, et al

We recommend at least 3 months