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To compare the end products of these processes to the original inocula and CWD field isolates, we will analyze their biochemical properties on Western blot using specific antibodies, their conformational stability, and cellular tropism

In some experiments, we introduced blockers to the wells after loading with Fluo-4 AM and waited for 15 min before taking readings

[4,5] Disturbances in insulin signaling, such as hypoinsulinemia, hyperinsulinemia, or insulin resistance, can impair neuronal homeostasis, disrupt gamma-aminobutyric acid (GABA)-glutamate equilibrium, trigger oxidative stress, and promote inflammatory cascades, ultimately lowering the seizure threshold

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It mimics the natural GLP-1 hormone, which is released from the gut after eating and plays a central role in appetite regulation and blood sugar control