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The high lipophilicity was also associated with off-target pharmacology such as inhibition of the human ether-a-go-go-related gene (hERG) ion channel (IC 50 = 5.6 M, Table S4)

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mRNA levels were determined using TaqMan probes for fatty acid binding protein 4 ( Fabp4 , Mm00445878_m1), perilipin-2 ( Plin2 , Mm00475794_m1), aryl hydrocarbon receptor nuclear translocator-like protein 1 (Arntl, Mm00500223_m), semaphorin 3 C ( Sema3c , Mm00443121_m1) and hypoxanthine phosphoribosyltransferase 1 ( Hp rt, Mm03024075_m1) in custom-made low density array cards (Thermo Fisher) or in single assays on a QuantStudio 7 Real-Time PCR system