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Most patients prefer injecting in the evening because any nausea or GI effects tend to peak four to eight hours post-injection, meaning they occur during sleep

Theres no washout period, no bridging strategy, and no need to retitrate from the beginning

Real-time PCR was performed using the QuantStudio 3 real-time PCR system (Invitrogen) and specific primers (Table 1

Youll also have the opportunity to ask a few questions of your own, so be sure to raise any concerns or uncertainties you may have about the process

Unlike CuAAC, which is limited to terminal alkynes, RuAAC expands the scope by allowing both terminal and internal alkynes to participate in the reaction