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June 04, 2021 Wilding JPH, Batterham RL, Calanna S, Davies M, Van Gaal LF, Lingvay I, McGowan BM, Rosenstock J, Tran MTD, Wadden TA, Wharton S, Yokote K, Zeuthen N, Kushner RF

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This observation also highlights the complexity that modifying a residue within a cholesterol binding site might have on the overall pattern of receptor interactions with cholesterol and other plasma membrane lipids, as increased segregation to cholesterol-rich lipid rafts occurs despite an overall reduction in total cholesterol binding under vehicle conditions observed for the V229A mutant receptor