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The following day, the culture was concentrated 20-fold and spread on PA14 slow-killing plates, then dried under a hood
Brain GLP-1/IGF-1 Signaling and Autophagy Mediate Exendin-4 Protection Against Apoptosis in Type 2 Diabetic Rats

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Part of each tissue was fixed in 4% paraformaldehyde for morphological examination, and the rest was frozen in liquid nitrogen and preserved at -80 for later experiments
