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The medium was discarded, replaced with fresh medium containing 1, 5, 10 M ITP, or 2 nM insulin, or a mixture of ITP (10 M) and insulin (2, 10, 50 nM), and the cells were incubated at 37 C for 10 min

Fractions with a purity of greater than 95% were pooled and lyophilized

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