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The resin was washed by 20 column volumes of wash buffer A [20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.05% (w/v) DDM, 0.01% (w/v) CHS and 30 mM imidazole] and 10 column volumes of wash buffer B [20 mM HEPES, pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.02% (w/v) DDM, 0.01% (w/v) CHS and 50 mM imidazole], followed by incubation with Fab7F38 at a molar ratio of 1: 1.5 in 3 mL wash buffer C [20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.01% (w/v) DDM, 0.01% (w/v) CHS and 20 mM imidazole] for 3 h at 4 C

Brain imaging adds a physical layer: research by Jastreboff and colleagues found that when people with obesity see food cues, the brain's reward and craving regions light up more intensely

Kessler says drugmakers should do more to inform patients about the risks of developing eating disorders, and gastroparesis a chronic condition where gastric emptying slows down significantly

Salads are great, but they're far from the only option

The ~15% weight loss observed with semaglutide approaches the 2030% weight loss achieved through bariatric surgery