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Retrograde Labeling of GCs The detailed procedure were based on previous work [15, 16]
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It decreases systemic vascular resistance, pulmonary vascular pressure, and arterial pressure, ultimately reducing both preload and afterload, thereby decreasing the workload on the heart
Per-Batch HPLC + Disulfide Bond Verification Because the lipolytic conformation depends on an intact Cys7-Cys14 disulfide bond, every released lot is checked both by HPLC for peptide purity and by non-reducing LC-MS or Ellman's reagent analysis for disulfide bond integrity
