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For INS-1 832/3, Silencer Select siRNA (Thermo Fisher Scientific) targeting rat Arrb1 (ID: 129662) and Arrb2 (ID: 129665), or negative control siRNA, were used

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Contrasting the latter, loss of liver Fgf21 expression did not attenuate liraglutide-induced weight loss in mice fed a high fat (60%), low carbohydrate diet, further suggesting that the contribution of Fgf21 to weight loss by liraglutide is dependent on dietary carbohydrate content

All samples were then processed again using data-independent acquisition, in this case using 20 ppm and 15 ppm for precursor and fragment ion tolerance, respectively, against the prefabricated spectral library of 69,797 precursors to maximize discovery, precision, and reproducibility of the proteomic spectrum