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Samples were fixed with 4% PFA at room temperature for 30 min, permeabilized with 0.1% Triton-100 at room temperature for 15 min, and blocked with 5% bovine serum albumin (BSA) solution at 37 C for 1 h

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If the migraine benefit comes primarily from GLP-1 receptor activation, then semaglutide should theoretically offer similar benefits

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