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Stable CHOFlpIn expression cell lines were selected using 600 g/ml hygromyocin B, and maintained in DMEM supplemented with 5% (V/V) FBS (Invitrogen) at 37 C in 5% CO 2

Findings remained consistent across studies, reinforcing that semaglutide does not appear to negatively impact mental health in individuals without existing psychiatric conditions

Staying well-hydrated, eating small frequent meals, and avoiding high-fat foods can help manage gastrointestinal symptoms during this period

These medications are most effective when used alongside a structured approach to diet and physical activity

Age-appropriate skincare, sun protection, and lifestyle changes support skin throughout and post weight loss
