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RNA isolation and qRTPCR analysis Total RNA was extracted using TRIzol (Takara Bio, Tokyo, Japan) and quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed using Hieff qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) according to the manufacturers instructions

However, the platform does not routinely integrate peptide-pathway genetic testing into its decision framework, instead relying on clinical indicators and patient-reported outcomes to guide medication selection

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The coming 1824 months will see both medications available simultaneously