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To generate mutations at the APTF-2 target site in the glp-1 promoter we injected the following mix: Cas9 protein (5 g), tracrRNA (0.4 g/l), crRNA (aatgggcggagtatgccacc) (0.4 g/l), ssDNA oligo repair donor (1 g/l), myo-2::mCherry plasmid (4 ng/l) and brought to a 20 l volume with nuclease-free water

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Recording the date, injection site, any sensations experienced, and overall comfort rating creates a useful reference

One study reported that total plasma bile acid concentrations increased twofold after RYGB but decreased after AGB, despite similar weight loss (173)

This can have serious health consequences