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After separation, proteins were transferred to the nitrocellulose membrane at 80 mA and 4 C overnight

Under the current definition, ENDS include any electronic device that, through an aerosolized solution, delivers nicotine, flavor, or any other substance to the user inhaling from the device

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Smoking and vascular endothelial function Many studies have shown that the vascular endothelium-dependent relaxation response is impaired in smokers and in experimental animal models [42,43,44,45,46,47,48, 54, 55]