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The test can determine recent nicotine exposure, such as smoking or using nicotine products

After curing, leaves ferment to balance their chemical composition and remove harshness, then get sorted, blended, and cut into fine strips based on the intended product

10.1016/s0376-8716(96)01315-4 40 KeyesK

Dai J, Dong A, Xiong G, Liu Y, Qiu D (2020) Production of highly active extracellular amylase and cellulase from Bacillus subtilis ZIM3 and a recombinant strain with a potential application in tobacco fermentation

A 6-month systems toxicology inhalation study in ApoE / mice demonstrates reduced cardiovascular effects of E-vapor aerosols compared with cigarette smoke