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Primary and secondary sequences of every sample were analyzed using the Mutation surveyor (Version 5.2.0), BioEdit sequence alignment editor version 7.2.5, and Unipro UGENE (Version 50.0) software

All of those effects on the body add together to put smokers at a higher risk of many different medical conditions, including: Cancer Heart Disease Stroke COPD, Asthma Type 2 Diabetes Vascular Disease Eye Diseases Difficulties Conceiving (affecting both sexes) Reproductive health problems Birth Preterm Rheumatoid arthritis Smoking is a major risk factor for nearly every type of cancer

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