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The dried pellets were resuspended in 50 L of 50 mM TEAB then reduced with the addition of 1 L of 500 mM tris(2-carboxyethyl)phosphine (Thermo Scientific, Rockford, IL) and incubated at 37 C for 1 h
Bear in mind that you dont need to smoke in order to develop smoking-related cancers
If you identify smoking damage after a tenant moves out, you may need to bill them for excessive cleaning costs as per the lease agreement
doi: 10.1007/s001010050429
