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Once consumed, nicotine is quickly absorbed into the bloodstream, reaching the human brain in mere seconds and triggering a surge in dopamine levels

Detecting this disease early can significantly improve patient outcomes, yet many people dont realize they are at risk until symptoms become severe

RNA isolation and real-time PCR Total RNA from cells exposed to extracts for 48 h was extracted using TRIzol reagent (Invitrogen) and quantified using a nano-drop followed by Reverse Transcriptase PCR using random primers per manufacturers recommendations

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