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Northern blot hybridization Total RNA was extracted from plant tissue using the TRIzol reagent (Invitrogen)

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CTSmoke pours harmlessly from a tobacco barn off Highway 12 in Cheatham County, a sign of tobacco curing, not a fire.Chris Smith / The Leaf-ChronicleSmoke pours harmlessly from a tobacco barn off Highway 12 in Cheatham County, a sign of tobacco curing, not a fire.Chris Smith / The Leaf-ChronicleSmoke pours harmlessly from a tobacco barn off Highway 12 in Cheatham County, a sign of tobacco curing, not a fire.Chris Smith / The Leaf-ChronicleTobacco hanging in a barn ready for smoking.ContributedThe fires inside a tobacco barn.ContributedSmoke drifts from a tobacco barn in Montgomery County.ContributedFeatured Weekly Ad