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Norepinephrine increases alertness and arousal, while acetylcholine enhances cognitive functions such as memory and learning

The cells were counted and resuspended in FACS buffer (PBS + 0.5% bovine serum albumin + 0.01% NaN 3 ) at 4 C at a concentration of 1 10 7 cells mL 1 in a sterile FACS tube (5-mL polystyrene round-bottom tube)

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A 19 min gradient using 5 mM ammonium acetate in ultra-pure water (UPW):methanol (1:1) with 0.1% formic acid (mobile phase A), 5 mM ammonium acetate in methanol with 0.1% formic acid (mobile phase B) and 10 mM ammonium acetate in isopropanol with 0.1% formic acid (mobile phase C) was used