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Electric machines are faster and more convenient for those who need to roll multiple cigarettes at once, but they may not offer the same level of control that manual machines provide
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ISBN 978-2354030506
Because non-CpG sites can be methylated in mtDNA, which may reduce the PCR primer binding efficiency [45], we verified PCR product intensity on 1% agarose gel and inspected the peak heights in pyrosequencing readouts
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